Fig. 5: Correlation of CBX2 expression and autophagy, apoptosis, and EMT.
From: CBX2 identified as driver of anoikis escape and dissemination in high grade serous ovarian cancer
Utilizing the TCGA (HGSOC, Nature, 2011) dataset, CBX2 expression was correlated to mRNA expression of all genes and 5838 genes (CBX2-associated genes) were identified to have a Spearman correlation of greater than r = 0.15. a The 5838 genes were cross-referenced with published gene sets for “Apoptosis”, “Autophagy”, and “Epithelial to Mesenchymal Transition (EMT).” Percentage indicates overlap with gene lists. PEO1, OVCAR4, and OVCAR8 cells were grown in adherent (Adh) or suspension (Sus) for 7 days, RNA was extracted, and used for RT-qPCR against MYLK (b), NOG (c), and TNFSF10 (d). Statistical test = two-sided t-test. shControl and shCBX2 #1 and 2 PEO1, OVCAR4, and OVCAR8 cells were grown in suspension, RNA was extracted, and used for RT-qPCR against MYLK (e), NOG (f), and TNFSF10 (g). Statistical test = ANOVA. Error bars = S.E.M.
