Fig. 3: The cytosolic domain of SEMA6A induces apoptosis in lung cancer cell lines both in vitro and in vivo. | Oncogenesis

Fig. 3: The cytosolic domain of SEMA6A induces apoptosis in lung cancer cell lines both in vitro and in vivo.

From: The extracellular SEMA domain attenuates intracellular apoptotic signaling of semaphorin 6A in lung cancer cells

Fig. 3

a Schematic diagram of the SEMA6A constructs. 6A: full length. 6Aect: extracellular domain of SEMA6A. 6Acyto: cytosolic domain of SEMA6A. b Immunoblotting of lysates of A549 and H1299 cells overexpressing 6A-FL, 6Aect, 6Acyto, or empty vector (Ctrl). All constructs were histidine tagged. Loading control: actin. c and d Proliferation of A549 c and H1299 d cells overexpressing different truncations of SEMA6A. **P < 0.01, n = 4. e Colony formation of A549 and H1299 cells. **P < 0.01, n = 4. f Quantitative analysis of the apoptotic A549 and H1299. **P < 0.01, n = 4. g Tumor volume (left) and weight (right) in mice transfected with 6Aect-, 6Acyto-, or Ctrl-overexpressing H1299 cells. Inset: Tumor image from Ctrl-, 6Aect-, or 6Acyto-overexpressing H1299 xenografts. **P < 0.01, n = 5

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