Fig. 4: Reintroduction of the SEMA domain inhibits the apoptosis induced by 6AΔsema. | Oncogenesis

Fig. 4: Reintroduction of the SEMA domain inhibits the apoptosis induced by 6AΔsema.

From: The extracellular SEMA domain attenuates intracellular apoptotic signaling of semaphorin 6A in lung cancer cells

Fig. 4

a Schematic diagram of the 6AΔsema construct in comparison to 6A-FL. b Immunoblotting analysis of A549 and H1299 cells overexpressing His-tagged 6AΔsema. Loading control: actin. c Percentage of apoptotic A549 and H1299 cells overexpressing 6AΔsema. **P < 0.01, n = 4. d Percentage of apoptotic A549 and H1299 cells overexpressing 6AΔsema after addition of purified SEMA domain. **P < 0.01, n = 4 per indicated concentration. e Percentage of apoptotic A549 and H1299 cells overexpressing 6Acyto after external addition of purified SEMA domain. n = 4 for each concentration. *P < 0.05, **P < 0.01

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