Fig. 5: The cytosolic domain of SEMA6A binds to FADD. | Oncogenesis

Fig. 5: The cytosolic domain of SEMA6A binds to FADD.

From: The extracellular SEMA domain attenuates intracellular apoptotic signaling of semaphorin 6A in lung cancer cells

Fig. 5

a Immunoblot of caspase-8 cleavage products (p43/41 and p18) in A549 and H1299 cells overexpressing constructs of SEMA6A. b Immunoblotting analysis of p43/41 and p18 in H1299 cells overexpressing 6AΔsema. Co-immunoprecipitation of FADD and 6Acyto c, 6AΔsema d, and 6Aect e in 293T cells. Input: 5% of non-immunoprecipitated cell lysate. IgG: control IP with isotype-matched IgG antibody from rabbit. The input and immunoprecipitates were subjected to western blot analysis using anti-FADD and anti-His antibodies

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