Fig. 6: The cytosolic domain of SEMA6A induces apoptosis by interacting with FADD. | Oncogenesis

Fig. 6: The cytosolic domain of SEMA6A induces apoptosis by interacting with FADD.

From: The extracellular SEMA domain attenuates intracellular apoptotic signaling of semaphorin 6A in lung cancer cells

Fig. 6

a Immunoblotting analysis of silencing FADD in A549 and H1299 cells with two FADD shRNAs (shFADD #1 and #2) and empty lentiviral vector (Ctrl). b Percentage of apoptosis in A549 and H1299 cells overexpressing 6Acyto and transfected with shRNAs against FADD. **P < 0.01, n = 3. c Western blot analyses of A549 and H1299 cells overexpressing dominant-negative mutant of FADD (DN-FADD). The upper band is endogenous FADD and the lower band is DN-FADD. d Percentage of apoptosis in 6Acyto-overexpressing A549 and H1299 cells with DN-FADD overexpression. **P < 0.01, n = 3. Co-immunoprecipitation and quantification of FADD and 6AΔsema e and f or 6Acyto g and h in the presence of purified SEMA domain. **P < 0.01, n = 3

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