Fig. 2: CUL5 is required to maintain mitotic fidelity. | Oncogenesis

Fig. 2: CUL5 is required to maintain mitotic fidelity.

From: Cullin 5 is a novel candidate tumor suppressor in renal cell carcinoma involved in the maintenance of genome stability

Fig. 2

a Fluorescence microscopic analysis (left panel) and quantification (right panel) of abnormal mitotic cells after siRNA-mediated depletion of CUL5 in U-2OS/centrin-GFP cells (96 h). Insets show the spindle poles. Scale bar = 10 μm. Each bar represents mean plus standard error of at least two independent experiments with a minimum of 100 cells counted per experiment. b Flow cytometric analysis of U-2 OS/centrin-GFP cells following siRNA-mediated depletion of CUL5 (siCUL5) or control siRNA (siControl) for 72 h. Arrow indicates poly-/aneuploid cells. Asterisks indicate statistically significant differences (*p ≤ 0.05)

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