Table 1 List of primers used for the study

From: Estrogen receptor-α regulation of microRNA-590 targets FAM171A1—a modifier of breast cancer invasiveness

Gene name

Primers used

FAM171A1

Forward 5′-CCTCGCGTTCATTTCCAGAG-3′

Reverse 5′-GGCGTTCCATTACTGCTCAG-3′

ESR1 (ERα)

Forward 5′-CCACCAACCAGTGCACCATT-3′

Reverse 5′-GGTCTTTTCGTATCCCACCTTTC-3′

HER2

Forward 5′-AACTGCACCCACTCCTGTGT-3′

Reverse 5′-TGATGAGGATCCCAAAGACC-3′

GAPDH

Forward 5′-CACCAGGGCTGCTTTTAACTCTGGTA-3′

Reverse 5′-CCTTGACGGTGCCATGGAATTTGC-3′

FAM(ChIP)-ERα1

Forward 5′-CCCAGCCAAGTCTGGAGGT-3′

Reverse 5′-GGGCACAACTGAGCCTAGAAG-3′

FAM(ChIP)-ERα2

Forward 5′-GAGGGTCTTCTTTGCTGTGC-3′

Reverse 5′-ACCAGGTGCCATCCTAATGT-3′

PS2(ChIP)

Forward 5′-TTAGCTTAGGCCTAGACGGAATGG-3′

Reverse 5′-GACGACATGTGGTGAGGTCATCTT-3′

CYCLIN-D(ChIP)

Forward 5′-CATTCAGAGGTGTGTTTCTCCC-3′

Reverse 5′-CTCAGCGACTGCATCTTCTTTC-3′

FAM-CHIP-ERα3

Forward 5′-GAGGGTCTTCTTTGCTGTGC-3′

Reverse 5′-TCAGGAGGAATGGTACTTGGA-3′

Snail

Forward 5′-AATCGGAAGCCTAACTACAGCGAG-3′

Reverse 5′-CCTTGGCCTCAGAGAGCTGG-3′

Slug

Forward 5′-AGATGCATATTCGGACCCAC-3′

Reverse 5′-CCTCATGTTTGTGCAGGAGA-3′

Vimentin

Forward 5′-GACAATGCGTCTCTGGCACGTCTT-3′

Reverse 5′-TCCTCCGCCTCCTGCAGGTTCTT-3′

E-cadherin

Forward 5′-GAAGGTGACAGAGCCTCTGGAT-3′

Reverse 5′-GATCGGTTACCGTGATCAAAATC-3′

N-cadherin

Forward 5′-ACAGTGGCCACCTACAAAGG-3′

Reverse 5′-CCGAGATGGGGTTGATAATG-3′

ZEB1

Forward 5′-GGCAGAGAATGAGGGAGAAG-3′

Reverse 5′-CTTCAGACACTTGCTCACTACTC-3′

Pre-miR-590

Forward 5′-GGCTATCCTCTCAGAGTGACATTT-3′

Reverse 5′-GCTTTATCAGGTTATGTTGCATGGT-3′

miR-590-5p

Forward 5′-AAAGGATCCGTGGGTGAGTATGGGAGGGA-3′

Reverse 5′-AAAAAGCTTGCCAAGCTAAGCCAAGGGTA-3′

miR-590 promoter

Forward 5′-CTTTATGCTGACCTGCTTG-3′

Reverse 5′-TCTCCTTGTTGCCTGATT-3′

miR-590(ChIP)

Forward 5′-TCAAGCGATTCTCCTGC-3′

Reverse 5′-AAGCGTCGTTTACTCTGC-3′

SG6K(ChIP)

Forward 5′-TGCAAGGAGAACATTGAACACAA-3′

Reverse 5′-CCGGCGTGCCACAGA-3′

DNAMT3A(ChIP)

Forward 5′-AGGAACCTAGAGCCCTGAGC-3′

Reverse 5′-GTGAGTTCCCCGTACCTTGA-3′

FAM −3′-UTR

Forward 5′-AAACTCGAGAGACCCACCTGACTGTGGAATATAA-3′

Reverse 5′-AAAGCGGCCCCTTTTCTTCAGAGTTTATTTTGAA-3′

U6 snRNA

Forward 5′-CTCGCTTCGGCAGCACA-3′

Reverse 5′-AACGCTTCACGAATTTGCGT-3′

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