Fig. 3: CDCA5 knockdown suppresses the growth of cultured CRC cells. | Oncogenesis

Fig. 3: CDCA5 knockdown suppresses the growth of cultured CRC cells.

From: Cell division cycle associated 5 promotes colorectal cancer progression by activating the ERK signaling pathway

Fig. 3

a Western-blot analysis of CDCA5 in HCT116 (left panel) and HT-29 (right panel) cells after transduction with sh-CDCA5 vs. sh-Ctrl lentivirus. The integrated band density was determined using the ImageLab Software, and GAPDH as the internal control (*P < 0.05). b After transduction with sh-CDCA5 vs. sh-Ctrl lentivirus for 72 h, cell number was counted using trypan blue exclusion (*P < 0.05, vs. sh-Ctrl). Left: HCT116; Right: HT-29. c Cell viability of HCT116 and HT-29 cells was determined using CCK-8 assay after transduction with sh-CDCA5 vs. sh-Ctrl lentivirus. Data were normalized to viability on day 1 and shown as fold change. *P < 0.05 vs. sh-Ctrl. d Cell survival was analyzed by colony formation assay in HCT116 or HT-29 cells after transduction with sh-CDCA5 or sh-Ctrl lentivirus. Representative images of colonies after CDCA5 knockdown are shown. *P < 0.05 vs. sh-Ctrl. All experiments were performed in triplicate

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