Fig. 4: Fbw7 reduces the function of XBP1s activating oncogenic signals.

a HCT116 cells were co-transfected with the Myc-luc reporter, XBP1s expressing vector, and Flag-Fbw7 expressing vector and then analyzed for luciferase activity at 48 h after transfection. b HCT116 cells were co-transfected with the AP1-luc reporter, XBP1s expressing vector, and Flag-Fbw7 expressing vector and then analyzed for luciferase activity after 48 h. c HCT116 cells were co-transfected with the NF-κB -luc reporter, XBP1s expressing vector, and Flag-Fbw7 expressing vector and then analyzed for luciferase activity after 48 h. d At 10 days after seeding HCT116 control/XBP1 overexpressing/Fbw7 knock down/both XBP1 overexpressing and Fbw7 knock down cells, they were stained with 5% crystal violet. e Number and size of colonies from three independent experiments. f Scratched HCT116 XBP1-overexpressing cells or HCT116 XBP1-overexpressing cells with Fbw7-null were observed until 48 h using incucyte. g Statistical analysis of relative wound density. h Proliferation rate of HCT116 XBP1-overexpressing cells or HCT116 XBP1-overexpressing cells with Fbw7-null were detected with Incucyte until 96 h. Data are expressed as means ± SD (n = 3). *p < 0.05, **p < 0.01, and ***p < 0.001