Fig. 5: XBP1s downregulates Fbw7 transcription.
From: A negative feedback loop between XBP1 and Fbw7 regulates cancer development
a–c HCT116 cells were co-transfected with XBP1s (0, 0.5, and 1 μg) and FLAG-Fbw7 expressing vector. d–f HCT116 cells were transfected with XBP1s (0, 0.1, 0.5, and 1 μg). Exogenous and endogenous Fbw7 mRNA was detected by reverse transcriptase PCR (RT-PCR) and real-time PCR (qPCR). g, h HCT116 cells were transfected with XBP1s (0, 0.1, 0.5, and 1 μg). Endogenous Fbw7 protein was detected by western blotting. Western blotting was carried out with indicated antibodies. (I) Effect of tunicamycin (TM) induced endogenous XBP1s on Fbw7 was detected by qPCR. Data are expressed as means ± SD (n = 3). *p < 0.05, **p < 0.01, and ***p < 0.001
