Fig. 3: Restoration of p53 in lung adenocarcinomas leads to potent immune inflammatory response in the lung.

Cell suspensions from Rag1^-/- mouse lungs bearing orthotopically transplanted KPr adenocarcinomas were treated with corn oil (Control) or tamoxifen (Restored) and subjected to multiparameter flow cytometry. a Cells gated to include live singlets are plotted on histograms for CD45 and quantified at right. b CD45^pos. cells from a plotted on F4/80 X SSC. F4/80^pos. cells gated and quantified at right. c CD45^pos. cells from a plotted on CD11b X F4/80. CD11b^neg.;F4/80^pos. alveolar macrophages at bottom left and CD11b^pos.F4/80^pos. CD11b^pos. macrophages gated and quantified at bottom right. d CD11b^pos. cells from a plotted on Ly6G X Ly6C. CD11b^pos.Ly6G^pos. neutrophils ^and CD11b^pos.Ly6C^pos. monocytes cells are gated and quantified at right. Analysis of significance between control and restored groups was performed by t-test