Fig. 3: In vitro oncogenic activity of NAF1 in glioma cells. | Oncogenesis

Fig. 3: In vitro oncogenic activity of NAF1 in glioma cells.

From: Increased expression of NAF1 contributes to malignant phenotypes of glioma cells through promoting protein synthesis and associates with poor patient survival

Fig. 3

a Western blot analysis was performed to verify NAF1 depletion by two different siRNAs (si-NAF1-654 and si-NAF1-927) in SF295 and U87 cells with GAPDH as an endogenous control. b Cell viability upon NAF1 knockdown in SF295 and U87 cells was determined by the MTT assay. *P < 0.05; **P < 0.01 (n = 5). c Left panels show the representative images of colony formation in soft agar in SF295 and U87 cells upon NAF1 knockdown. Scale bars, 50 μm. Quantitative analysis of colony numbers (right panels). *P < 0.05; **P < 0.01 (n = 5). d Measurement of cell apoptosis in SF295 and U87 cells by flow cytometry. *P < 0.05; **P < 0.01 (n = 3). e The left panels are representative images of migrated/invaded cells upon NAF1 knockdown. The quantitative analysis of the number of migrated/invaded cells (right panels). Scale bars, 100 μm. *P < 0.05; **P < 0.01; ***P < 0.001 (n = 5). f Validation of ectopic expression of NAF1 in SF295 and U87 cell lines by western blot analysis with GAPDH as a loading control. g Cell viability upon NAF1 overexpression in SF295 and U87 cells was detected by the MTT assay, *P < 0.05; **P < 0.01 (n = 5). h The representative images of colony formation in soft agar upon NAF1 overexpression in SF295 and U87 cells (left panels). Scale bars, 50 μm. The right panels are the quantitative analysis of colony numbers. *P < 0.05; **P < 0.01 (n = 5). i The left panels are representative images of migrated/invaded cells upon NAF1 overexpression. Quantitative analysis of the number of migrated/invaded cells (right panels). Scale bars, 100 μm. Showing data as mean ± SD. *P < 0.05; **P < 0.01 (n = 5)

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