Fig. 5: NAF1 promotes the expression of c-Myc, NRF2, TERT, POLR1A, and POLR2A in glioma cells. | Oncogenesis

Fig. 5: NAF1 promotes the expression of c-Myc, NRF2, TERT, POLR1A, and POLR2A in glioma cells.

From: Increased expression of NAF1 contributes to malignant phenotypes of glioma cells through promoting protein synthesis and associates with poor patient survival

Fig. 5

a, b Protein expression levels of c-Myc, NRF2, and TERT upon NAF1 knockdown or ectopic expression in SF295 and U87 cells were determined by western blot analysis with GAPDH as a loading control. c, d The qRT-PCR assay was carried out to detect mRNA expression of c-Myc, NRF2, and TERT upon depletion or overexpression of NAF1 in SF295 and U87 cells. β-Actin was used as an endogenous control. Data were shown as mean ± SD. **P < 0.01; ***P < 0.001 (n = 3). e, f Western blot analysis was used to evaluate protein expression of POLR1A and POLR2A upon knockdown or ectopic expression of NAF1 in SF295 and U87 cells. GAPDH was used as an endogenous control. The western blot is representative of three independently preformed experiments

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