Fig. 1: CHMP7 and VPS4 depletion cause general nuclear envelope disorganization. | Oncogenesis

Fig. 1: CHMP7 and VPS4 depletion cause general nuclear envelope disorganization.

From: ESCRT-III is necessary for the integrity of the nuclear envelope in micronuclei but is aberrant at ruptured micronuclear envelopes generating damage

Fig. 1

a Examples of the honeycomb and herniated Lamin A and B phenotypes in HeLa cells treated with CHMP7 sRNAi for 48 h. Arrowheads indicate chromatin protruding out the nucleus in herniated cells. b Quantification of Lamin A and B defects in Hela cells treated with the indicated sRNAi (minimum of 300 cells scored per condition). c Examples of CHMP7 foci in HeLa cells treated with VPS4 and NT-control sRNAi for 48 h and associated with LAP2 or Lamin A foci (LAP2 and Lamin A enriched, arrowheads) or associated with Lamin B holes (HeLa M). d Average CHMP7 foci number per nucleus in HeLa (n = 305), HeLa M (n = 222), and U2OS (n = 257) cells treated with VPS4 sRNAi for 48 h. e Quantification of CHMP7 foci in relation to LAP2, Lamin A/C, or Lamin B, in HeLa cells treated with VPS4 sRNAi for 48 h (minimum of 400 foci for each condition). f Deconvolved widefield image of CHMP4B co-localization with CHMP7 at nuclear envelope foci in HeLa cells transfected with VPS4 sRNAi for 48 h (top panel) and CHMP7/CHMP4B distribution in control cells (bottom panel). g Percentage of HeLa cells containing at least one large accumulation of CHMP7 (focus) following VPS4 sRNAi treatment at 24, 48, and 72 h post transfection (minimum of 200 nuclei scored for each condition). h Number of nuclear CHMP foci accumulating in HeLa M cells under the indicated sRNAi treatments. (minimum of 100 nuclei scored for each condition). Averages and SEM are shown (N = 3). Scale bars represent 10 μm

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