Fig. 3: ESCRT-III accumulates on micronuclei and is recruited by CHMP7. | Oncogenesis

Fig. 3: ESCRT-III accumulates on micronuclei and is recruited by CHMP7.

From: ESCRT-III is necessary for the integrity of the nuclear envelope in micronuclei but is aberrant at ruptured micronuclear envelopes generating damage

Fig. 3

a Untreated HeLa cells showing accumulations of CHMP7 and CHMP4B at a micronucleus (arrowhead). Scale bars 10 µm. b U2OS, HeLa, and HeLa M cell lines were transfected with non-targeting or VPS4A and B sRNAi for 48 h, and stained for CHMP7. Micronuclei were scored for presence or absence of CHMP7 (minimum of 400 micronuclei scored per treatment). c Percentage of micronuclei in HeLa cells transfected with the indicated sRNAi for 48 h displaying enriched CHMP7 or CHMP4 (minimum 200 micronuclei scored per treatment). d Confocal microscopy in STED mode of a micronucleus in a control HeLa cell containing CHMP7 and CHMP4B accumulations. Scale bars represent 1 µm. e Confocal microscopy in STED mode of the micronucleus in d showing z-slicing across 335 nm starting from the top going towards the inside of the micronucleus. Scale bar 1 μm. Averages and SEM are shown (N = 3)

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