Fig. 2: KIT-induced autophagy sustains cell proliferation and cell survival. | Oncogenesis

Fig. 2: KIT-induced autophagy sustains cell proliferation and cell survival.

From: Oncogenic KIT mutations induce STAT3-dependent autophagy to support cell proliferation in acute myeloid leukemia

Fig. 2

a Impact of pharmacological inhibition of KIT on cell proliferation. TF-1 and TF-1 KITD816V cells were treated with PKC412 at 1 µM for 3 days and cell proliferation was evaluated by Trypan Blue exclusion counting (n = 3 ± SEM). b–e Consequences of invalidation of ATG12 and VPS34, two key autophagic proteins. TF-1 and TF-1 KITD816V cells were transduced with inducible shRNA against ATG12 or VPS34. Target proteins were invalidated after treatment with doxycycline (1 µg/mL). Effects of shRNA on cell proliferation (b), measured by Trypan blue exclusion (n = 3 ± SEM) and cell death (c) assessed after 7-AAD staining and flow cytometry (n = 3 ± SEM). d, e Colony-forming cell (CFC) assays. d shRNA-expressing cells were grown in methylcellulose medium in the presence of vehicle or doxycycline (5 µg/mL). e Quantification of colonies (n = 3 ± SEM)

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