Fig. 5: PR55α is essential for maintaining YAP stability. | Oncogenesis

Fig. 5: PR55α is essential for maintaining YAP stability.

From: PR55α regulatory subunit of PP2A inhibits the MOB1/LATS cascade and activates YAP in pancreatic cancer cells

Fig. 5

Cytoplasmic and nuclear extracts were isolated using the NE-PER™ Nuclear and Cytoplasmic Extraction Reagents (Fisher Scientific) and analyzed for YAP protein expression. a α-tubulin and Lamin A/C were used as the cytoplasmic and nuclear markers, respectively. PR55α in cytoplasm and nucleus was analyzed by Western blot analysis. b To inhibit cellular proteasome activity, CD18/HPAF cells transduced with Control-shRNA or PR55α-shRNA were treated with MG132 (25 μM) for the indicated hours. Cytoplasmic and nuclear extracts were isolated from the treated cells and analyzed for YAP expression by immunoblotting. α-tubulin and Lamin A/C served as the internal controls for cytoplasmic and nuclear extract, respectively. c To test the effect of PR55α on YAP protein stability, Control-/PR55α-shRNA-transduced CD18/HPAF cells were treated with CHX (15 μg/ml) to halt protein synthesis for the indicated hours, cytoplasmic and nuclear extracts were isolated from the treated cells, and YAP protein levels analyzed by immunoblotting. α-tubulin and Lamin A/C served as internal controls for cytoplasmic and nuclear extract, respectively

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