Fig. 4: BRG1 regulates LTBP2 transcription in lung cancer cells.

a Wild type and mutant CCNB1 promoter-luciferase constructs were transfected into LLC cells with or without BRG1 followed by exposure to 1% O₂ for 24 h. Luciferase activities were normalized by both protein concentration and GFP fluorescence. b HA-tagged BRG1 and His-tagged Sp1 were transfected into HEK293 cells. Co-immunoprecipitation was performed with indicated antibodies. c LLC cells were exposed to 1% O₂ and harvested 24 h later. Re-ChIP assay was performed with indicated antibodies. d LLC cells were transfected with siRNA targeting Sp1 or SCR before exposure to 1% O₂ for 24 h. ChIP assays were perormed with anti-BRG1 or IgG. Inset, knockdown efficiency of Sp1. e LLC cells were treated with or without mithramycin before exposure to 1% O₂ for 24 h. ChIP assays were perormed with anti-BRG1 or IgG