Fig. 5: BRG1 recruits KDM3A to regulate transcription.

a–d LLC cells were treated with a BRG1 inhibitor and exposed to 1% O₂ for 24 h. ChIP assays were performed with indicated antibodies. e HA-tagged BRG1 and Myc-tagged KDM3A were transfected into HEK293 cells. Co-immunoprecipitation was performed with indicated antibodies. f LLC cells were exposed to 1% O₂ and harvested 24 h later. Re-ChIP assay was performed with indicated antibodies. g–j LLC cells were transfected with siRNA targeting KDM3A or SCR before exposure to 1% O₂ for 24 h. Gene expression levels were examined by qPCR and Western. MTT assay and wound healing assay were performed and quantified as described in Methods