Fig. 6: BRG1 directly activates KDM3A transcription.

a, b LLC cells were transfected with HA-tagged BRG1 or an empty vector (EV) before exposure to 1% O₂ for 24 h. Gene expression levels were examined by qPCR and Western. c, d LLC cells were transfected with siRNA targeting BRG1 or SCR before exposure to 1% O₂ for 24 h. Gene expression levels were examined by qPCR and Western. e, f LLC cells were treated with a BRG1 inhibitor and exposed to 1% O₂. Gene expression levels were examined by qPCR and Western. g Wild type and mutant KDM3A promoter-luciferase constructs were transfected into LLC cells with or without BRG1 followed by exposure to 1% O₂ for 24 h. Luciferase activities were normalized by both protein concentration and GFP fluorescence. h LLC cells were exposed to 1% O₂ and harvested at indicated time points. ChIP assays were performed with anti-BRG1. i HA-tagged BRG1 and GFP-tagged HIF-1α were transfected into HEK293 cells. Co-immunoprecipitation was performed with indicated antibodies. j LLC cells were exposed to 1% O₂ and harvested 24 h later. Re-ChIP assay was performed with indicated antibodies. k A schematic model