Fig. 3: XIAP BIR domains promote transactivation of E2F1 and Sp1 in human BC cells. | Oncogenesis

Fig. 3: XIAP BIR domains promote transactivation of E2F1 and Sp1 in human BC cells.

From: XIAP Interaction with E2F1 and Sp1 via its BIR2 and BIR3 domains specific activated MMP2 to promote bladder cancer invasion

Fig. 3

a Schematic representation of the transcription factor binding sites in the human miR-203 promoter-driven luciferase reporter. b The indicated cell extracts were subjected to Western blot to determine the functional transcription factors. β-Actin was used as a protein loading control. c, d T24T (Nonsense), T24T (shXIAP/Vector), and T24T (shXIAP/ΔRING) cells were transfected with a Sp1-dependent luciferase reporter (c) or an E2F1-dependent luciferase reporter (d), together with pRL-TK. The results are presented as luciferase activity relative to that of the vector control transfectants. e ChIP assay was performed using anti-E2F1 or anti-Sp1 antibody to detect the interaction between E2F1 or Sp1 and the miR-203 promoter.

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