Fig. 2: Effects of Sec62 on the migration and invasive potential of Huh7 HCC cells.

Huh7 cells were transfected with LV- Sec62 shRNA, LV-Sec62 or LV-mock. Then, stable clones were selected. a The motility of these cells was evaluated by a monolayer wounding assay. Transwell assay analysis of the migration (b) and invasion (c) abilities of the indicated cells. The number of cells that invaded through the filter into the lower compartment was determined using a colorimetric crystal violet assay. Data are presented as the means ± SD of at least three independent experiments and were compared to the amounts of invaded cells from negative control transfected cells. *P < 0.01 versus the negative control (NC) group (Student’s t test).