Fig. 5: N-glycosylated SGK196 suppresses metastasis by inhibiting the PI3K/AKT /GSK3β signaling pathway.
From: N-glycosylated SGK196 suppresses the metastasis of basal-like breast cancer cells
a Western blotting analysis showing that phosphorylation of AKT and GSK3β and Snail expression were elevated after knockdown of SGK196 in MDA-MB-231 and BT-549 cells. Densitometric analysis was performed for AKT and GSK3β phospholyation and for the levels of Snail. b Western blotting analysis showing that the increase of p-AKT, p-GSK3β, and Snail caused by knockdown of SGK196 was reversed by re-expression of SGK196-WT, but not SGK196-4NQ, densitometric analysis was performed for AKT and GSK3β phospholyation and for the levels of Snail. c Western blotting analysis showing that the increase of p-AKT, p-GSK3β and Snail expression caused by knockdown of SGK196 was prevented by LY294002, an inhibitor of PI3K. Densitometric analysis was performed for AKT and GSK3β phospholyation and for the levels of Snail. d–g The MDA-MB-231 and BT-549 cells with depleted SGK196 were treated with 10 μM LY294002 and subjected to migration (d, e) and invasion assays (f, g). The data are presented as the mean ± S.D, **p < 0.01.
