Fig. 5: CXCL5-activated neutrophils promote GC cell migration and invasion by inducing EMT through IL-6 and IL-23. | Oncogenesis

Fig. 5: CXCL5-activated neutrophils promote GC cell migration and invasion by inducing EMT through IL-6 and IL-23.

From: CXCL5 promotes gastric cancer metastasis by inducing epithelial-mesenchymal transition and activating neutrophils

Fig. 5

a Transwell migration and Matrigel invasion assays for GC cells treated with conditioned medium of untreated (0-NCM) and CXCL5 (100 nM)-treated (100-NCM) neutrophils. b Western blot assays for the expression of EMT markers in GC cells treated with conditioned medium of untreated and CXCL5-treated neutrophils. c Transwell migration and Matrigel invasion assays for GC cells treated with conditioned medium of untreated (NCM) or TTCM-primed neutrophils (TNCM) in the presence or absence of CXCL5 neutralizing antibody. d ELISA assays for IL-6 and IL-23 concentrations in culture supernatants from CXCL5-primed neutrophils. e Transwell migration and Matrigel invasion assays for GC cells treated with conditioned medium of CXCL5-activated neutrophils in the presence or absence of IL-6 and IL-23 neutralizing antibodies. f Western blot assays for the expression of EMT markers in GC cells treated with conditioned medium of CXCL5-activated neutrophils in the presence or absence of IL-6 and IL-23 neutralizing antibodies.

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