Fig. 3: SCUBE3 silence inhibited U251 cell proliferation.
From: Reduction of SCUBE3 by a new marine-derived asterosaponin leads to arrest of glioma cells in G1/S
a Effects of lentivirus-mediated silencing of nine genes in U251 cells as examined using fluorescence microscopy. The representative pictures shown are from one of three independent experiments; b 5 days of continuous counting of the cell numbers by HCS (n = 3); c 5 days of continuous counting of the cell numbers fold change by HCS (n = 3, *p < 0.05 compared to blank-shctrl U251); d 5 days of continuous CCK-8 assays (n = 3, *p < 0.05 compared with blank-shctrl U251); e 5 days of continuous CCK-8 assays, fold change (n = 3, *p < 0.05 compared with blank-shctrl U251); f the efficiency of knockdown SCUBE3 by shRNA was verified in U251 cells by western blot at 96 h (n = 3; “+” represents positive, and “−” represents negative compared with blank-shctrl U251); g the efficiency of knockdown SCUBE3 by shRNA was verified in U251 cells by qPCR at 96 h (n = 3, ***p < 0.001 compared with blank-shctrl U251).
