Fig. 6: SCUBE3 knockdown arrested G1/S transition for CN-3 in U251 cells.
From: Reduction of SCUBE3 by a new marine-derived asterosaponin leads to arrest of glioma cells in G1/S
a 1.42 μM CN-3 arrested G1/S transition in U251 cells examined by flow cytometry (n = 3, ***p < 0.001 compared with normal U251); b knockdown and overexpression of SCUBE3 revealed SCUBE3-mediated G1/S transition in U251 cells, and SCUBE3 overexpression rescued the arrest induced by CN-3 or SCUBE3 silencing (n = 3, **p < 0.01 and ***p < 0.001 compared with blank-shctrl group); c in U251 cells, western blot results showed knockdown of SCUBE3 increased p53, p27, and p21 and decreased Akt, p-Akt, and E2F1. Remarkably, SCUBE3 knockdown and 1.42 μM CN-3 decreased p-Akt in U251 cells, while overexpression of SCUBE3 abolished this decrease (n = 3; “+” represents positive, and “−” represents negative).
