Fig. 2: OPN mediates the migration and invasion of lung cancer cells overexpressing SQS.
From: Squalene synthase promotes the invasion of lung cancer cells via the osteopontin/ERK pathway
a Left panel, OPN promoter activity assay in CL1-0/SQS cells. CL1-0/vector cells were used as controls, and GFP served as a transfection internal control. After 48 h, green fluorescence was detected in the cells, followed by treatment with firefly luciferase for luminescence determination. Middle panel, Expression of SPP1 (OPN) mRNA in CL1-0/SQS cells compared with CL1-0/Vector. Right panel, OPN protein levels in cells overexpressing SQS compared with the vector control. b Expression of the SPP1 (OPN) mRNA in CL1-5/shSQS cells compared with CL1-5/nonsilencing (NS) control. Data are presented as the means ± SDs; **P < 0.01. The significance was determined by Student’s t test. c Left panel, western blot analysis of SQS and OPN levels in CL1-0/SQS cells after OPN knockdown. Right panel, Invasion and migration capabilities of CL1-0/SQS cells after OPN knockdown. d, e Effects of OPN replenishment on the migration/invasion of SQS-silenced A549 and CL1-5 cells. SQS-silenced cells were incubated in the presence or absence of OPN (10 μg/mL) and subjected to migration (d) and invasion (e) assays. Data are presented as the means ± SDs; *P < 0.05, **P < 0.01. The significance was determined by Student’s t test.
