Fig. 2: HMGB1 derived from tumor cells is responsible for lung metastasis of breast cancer. | Oncogenesis

Fig. 2: HMGB1 derived from tumor cells is responsible for lung metastasis of breast cancer.

From: Tumor-derived HMGB1 induces CD62Ldim neutrophil polarization and promotes lung metastasis in triple-negative breast cancer

Fig. 2

a mRNA expression of HMGB1 in various organs of naive mice and 2-week tumor-bearing mice. b Elisa analysis of HMGB1 concentration in cytoplasm of various organs of naive mice and 2-week tumor-bearing mice. c Elisa analysis of serum HMGB1 concentration with tumor progression. d, e The protein level (d) and the mRNA level (e) of HMGB1 in different tumor cell lines. 4T1-shHMGB1, HMGB1 knockout 4T1 cell line; EMT6-HMGB1, HMGB1 overexpressing EMT6 cell line. f Representative image of primary tumors and spleens of 4-week tumor-bearing mice formed by different tumor cell lines inoculation. g H&E-stained lung sections (left) and quantification (right) of lung metastasis of 4-week tumor-bearing mice. h Survival of mice (n = 10 each) after different tumor cell lines inoculation (Kaplan–Meier test). i Schematic illustration (left) and representative bioluminescent imaging (middle) and lung bioluminescent quantification (right) of Balb/c mice injected with luciferase-expressing EMT6 cells observed in 2 h. Data are mean ± SEM of one representative experiments. Similar results were seen in three independent experiments. Unpaired Student’s t tests, ns not significant. *p < 0.05, **p < 0.01, ***p < 0.001. See also Fig. S2.

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