Fig. 3: Hypoxia regulates the release of HMGB1 from the nucleus into the cytoplasm. | Oncogenesis

Fig. 3: Hypoxia regulates the release of HMGB1 from the nucleus into the cytoplasm.

From: Tumor-derived HMGB1 induces CD62Ldim neutrophil polarization and promotes lung metastasis in triple-negative breast cancer

Fig. 3

a Representative images on HMGB1-stained primary tumor sections of 2-week tumor-bearing mice. b Cell immunofluorescent analysis of HMGB1 localization in 4T1 tumor cells cultured with normoxia or hypoxia for 24, 48, and 72 h, respectively. Cells were stained with HMGB1 (red), α-tubulin (green) and DAPI(blue). c Western blot analysis of HMGB1 in cytoplasmic and nuclear components of tumor cell lines under normoxic and hypoxic conditions. d Elisa analysis of HMGB1 concentration of 4T1 tumor cells cultured supernatant (TCCS) under normoxic and varying degrees of hypoxic conditions. e Elisa analysis of HMGB1 concentration of different TCCS under normoxic and hypoxic (72 h) conditions treated with or without α-lipoic acid and N-acetyl-L-cysteine (NAC). Data are mean ± SEM of one representative experiments. Similar results were seen in three independent experiments. Unpaired Student’s t tests, ns not significant. *p < 0.05, **p < 0.01, ***p < 0.001. See also Fig. S3.

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