Fig. 4: Hypoxia-released HMGB1 from primary tumor induce neutrophils to CD62L^dim.

a Quantification of neutrophils in BM, PB, and lung of 2-week tumor-bearing mice formed by different tumor cell lines inoculation. b Quantification of CD62L^dim neutrophils in PB and lung infiltrating neutrophils of 2-week tumor-bearing mice formed by different tumor cell lines inoculation. c Quantification of CD62L expression on neutrophils from BM neutrophils of naive mice treated with rHMGB1 for 4 h in vitro. d Flow analysis of CD62L^dim neutrophils in BM neutrophils of naive mice stimulated with different concentrated tumor tissue culture supernatant (TTCS) of tumor-bearing mice. e Flow analysis proportions of CD62L^dim neutrophils in BM neutrophils of naive mice stimulated with different concentrated TCCS under normoxic and hypoxic (72 h) conditions. f Flow analysis proportions of CD62L^dim neutrophils from naive mice BM induced with concentrated TCCS from normoxic or hypoxic (72 h) tumor cell with or without glycyrrhizic acid and ethyl pyruvate. Data are mean ± SEM of one representative experiments. Similar results were seen in three independent experiments. Unpaired Student’s t tests, ns, not significant. *p < 0.05, **p < 0.01, ***p < 0.001. See also Fig. S4.