Fig. 5: HMGB1 induces neutrophils to CD62L^dim neutrophils in breast cancer patients.

a Representative images of HMGB1 staining in TNBC. b Flow-cytometry gate strategy (left) and analysis proportions (right) of human PB CD62L^dim neutrophils detection. c The Pearson correlation between serum HMGB1 expression (by Elisa) and paired PB CD62L^dim neutrophils proportion of breast cancer patients (by Flow). d Change of CD62L expression on neutrophils from human PB treated with rHMGB1 for 4 h in vitro. e Change of CD62L expression on neutrophils from healthy donor PB treated with different patients’ serum. Data are mean ± SEM of one representative experiments. Similar results were seen in three independent experiments. Unpaired Student’s t tests, ns, not significant. *p < 0.05, **p < 0.01, ***p < 0.001. See also Fig. S4.