Fig. 7: “HMGB1-TLR2-CD62L^dim neutrophils-NETs” axis is responsible for lung metastasis of breast cancer.

a Representative bioluminescent imaging (left) and lung bioluminescent quantification (right) of Balb/c mice injected with luciferase-expressing EMT6 cells after CD62L^low neutrophils or rHMGB1 reinjection for 2 weeks. b, d Schematic illustration (b), representative bioluminescent imaging (c) and lung bioluminescent quantification (d) of Balb/c mice injected with luciferase-expressing 4T1 cells after multiple interventionrs for 2 weeks. e Flow analysis of PB and lung infiltrating CD62L^dim neutrophils in tumor-bearing mice after multiple intervention for 2 weeks. f, g Representative bioluminescent imaging (f) and lung bioluminescent quantification (g) for 4T1-shHMGB1 tumor-bearing mice in rescue experiments. 4T1-shH,4T1-shHMGB1; 4T1-shH-H, HMGB1 overexpressing 4T1-shHMGB1. h Flow analysis of PB and lung infiltrating CD62L^dim neutrophils in for 4T1-shHMGB1 tumor-bearing mice in rescue experiments. EP, Ethyl Pyruvate. Data are mean ± SEM of one representative experiments. Similar results were seen in three independent experiments. Unpaired Student’s t tests, ns not significant. *p < 0.05, **p < 0.01, ***p < 0.001. See also Fig. S6.