Fig. 3: MTHFD2 facilitates GC cell proliferation. | Oncogenesis

Fig. 3: MTHFD2 facilitates GC cell proliferation.

From: MiR-22, regulated by MeCP2, suppresses gastric cancer cell proliferation by inducing a deficiency in endogenous S-adenosylmethionine

Fig. 3

a IHC staining of MTHFD2 in clinical samples. b MTHFD2 protein levels in clinical samples. c MTHFD2 protein levels in GC cell lines and normal (GES-1). d MTHFD2 mRNA levels in GC cell lines and normal (GES-1). e MTHFD2 mRNA levels were detected by qRT-PCR in GC tissues and para-carcinoma tissues. f Bioinformatics analysis of MTHFD2 expressions in GC and para-carcinoma tissues based on TCGA data. g Colony formation assay 14 days after transfection with MTHFD2 siRNAs. h Cell proliferation was examined by MTT assay at 24, 48, and 72 h after transfection with MTHFD2 siRNAs. i Effect of silencing MTHFD2 on the GC cells cycle. j Effect of silencing MTHFD2 on GC cells apoptosis. k Effect of silencing MTHFD2 on cell cycle-related proteins. All the results are shown as the mean ± SD. n = 3, *p < 0.05, **p < 0.01, Student’s t test.

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