Fig. 5: Overexpression of MeCP2, MTHFD2, and MTHFR rescues miR-22-induced cellular phenotypes in GC cells.

a MTT assay of cell proliferation in AGS and MKN45 cells co-transfected with MeCP2 and miR-22. b Cell proliferation in AGS and MKN45 cells co-transfected with MTHFD2 and miR-22. c Cell proliferation in AGS and MKN45 cells co-transfected with MTHFR and miR-22. All the results are shown as the mean±SD. n = 3, *p < 0.05, **p < 0.01, Student’s t test. d Expression CDK4, P21, P16, and MeCP2 in cells co-transfected with MeCP2 and miR-22. e Expression CDK4, P21, P16, and MeCP2 in cells co-transfected with MTHFD2 and miR-22. f Expression CDK4, P21, P16, and MeCP2 in cells co-transfected with MTHFR and miR-22.