Fig. 3: FARP1 activates CDC42 and promotes filopodium formation in gastric cancer cell lines. | Oncogenesis

Fig. 3: FARP1 activates CDC42 and promotes filopodium formation in gastric cancer cell lines.

From: FARP1 boosts CDC42 activity from integrin αvβ5 signaling and correlates with poor prognosis of advanced gastric cancer

Fig. 3

a, b Active Rac1/CDC42/RhoA pulldown assay with serum stimulation in GSU and MKN7 cells infected with the FLAG-EGFP- or FLAG-FARP1–expressing lentivirus. c, d Immunofluorescence staining for actin (red) and DAPI (blue) with or without serum stimulation in GSU and MKN7 cells infected with the FLAG-EGFP- or FLAG-FARP1-expressing lentivirus. Magnification, ×400; scale bar, 50 μm in each of the three photos; magnification, ×200; scale bar, 100 μm in enlarge. SS serum stimulation. White arrow, filopodium formation. e, f Number and length of filopodia in the FLAG-EGFP- or and FLAG-FARP1-expressing cells. Values represent the means ± SD from six independent fields. *P < 0.05, **P < 0.01, ***P < 0.001; n.s. not significant (Student’s t test); RD relative density.

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