Fig. 4: Interaction of FARP1 with integrin αvβ5 and expression of integrin β5 in gastric cancer. | Oncogenesis

Fig. 4: Interaction of FARP1 with integrin αvβ5 and expression of integrin β5 in gastric cancer.

From: FARP1 boosts CDC42 activity from integrin αvβ5 signaling and correlates with poor prognosis of advanced gastric cancer

Fig. 4

a Gene sets identified as being related to FARP1 expression using GSEA. b Lysates from the FLAG-EGFP- or FLAG-FARP1-expressing GSU and MKN7 cells immunoprecipitated with the anti-FLAG antibody. Serum stimulation was applied before the cells were lysed. FLAG and integrin β5 were detected by western blotting. * indicate immunoglobulin heavy chain. c Correlation between FARP1 mRNA expression and integrin β5 mRNA expression in primary gastric cancer based on TCGA data. d Transwell migration assay in MKN74 cells transfected with NC Si, FARP1 Si1 or FARP1 Si2. e Numbers of migratory MKN74 cells transfected with NC Si, FARP1 Si1 or FARP1 Si2. In (d), 0.03% DMSO or 0.3 nM SB273005 diluted in 0.03% DMSO was applied when cells were inoculated onto the chamber. Magnification, ×100. Scale bar, 500 μm. e Values represent the means ± SD from six independent fields. ***P < 0.001; n.s. not significant (Student’s t test).

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