Fig. 2: miR-378a-5p (here abbreviated to miR-378) promotes in vitro tumor-progression-associated properties and induces uPAR expression in melanoma cells.
From: microRNA-378a-5p iS a novel positive regulator of melanoma progression
a In vitro cell migration and invasion of M14 and A375 melanoma cells transiently transfected with mimic miRNA scramble control (mimic Ctrl) or mimic miR-378. Values are expressed as number of migrated/invaded cells ± standard deviation. b Western blot analysis of MMP2 protein expression in M14 cells transiently transfected with mimic Ctrl or mimic miR-378. c Quantification of capillary-like structures formation in M14 cells transiently transfected with mimic Ctrl or mimic miR-378. Values are expressed as number of intersection points/field ± standard deviation. d VEGF secretion by M14 and A375 cells transfected with mimic Ctrl or mimic miR-378. Results are reported as fold over control. e Quantification of capillary-like structures formation in M14 cells transiently transfected with mimic Ctrl or mimic miR-378 incubated in the absence (no Ab) or presence of specific human VEGF- (anti-VEGF) or IL-8- (anti-IL-8) -neutralizing antibodies (0.2 ug/mL). Results are reported as number of intersection points/field (average ± SD). f qRT-PCR analysis of uPAR mRNA expression in M14 cells transfected with mimic Ctrl or mimic miR-378. The results are reported as fold induction ± SEM in cells transfected with mimic miR-378 relative to control ones. a, c-f Statistical analysis was performed applying t-test. ***p < 0.001, *p < 0.05. g Western blot analysis of uPAR protein expression in M14 and A375 cells transiently transfected with mimic miR-378 or with miR-378 inhibitor (anti-miR-378) and the relative miRNA scramble control (mimic Ctrl; anti-miR-Ctrl). b, g Representative images of one out of three independent experiments are reported. HSP72/73 and α-tubulin were used as loading and transferring control.
