Fig. 7: Transglutaminase-2 promotes EV-mediated pulmonary niche formation.
From: Transglutaminase-2 facilitates extracellular vesicle-mediated establishment of the metastatic niche
a Schematic illustration of the 3D niche assay. Human pulmonary fibroblasts (HPFs) were cultured on 3D scaffolds as described in the “Materials and methods” section and allowed to fill the open space of tessellated polymeric scaffolds. These cultures were treated with exosomes for another 3 weeks at which point MCF10-Ca1a responder cells stably expressing a firefly luciferase-dTomato fusion protein were added to the culture. b Growth of labeled Ca1a cells was longitudinally quantified by bioluminescence at the indicated time points. Data are mean relative luminescence units (RLU), normalized to the time zero (T0) reading, ±SE for three independent experiments completed in triplicate resulting in the indicated p values. c Representative fluorescent and brightfield merged images showing MCF10-Cala cell colonies (red) growing on the HPFs.
