Fig. 2: O-GlcNAcylation of PFKFB3 maintain cell cycle progression under hypoxia.

a–d SW1990 cells with indicated shRNA expression were cultured for 12 h (a, b), 24 h (c), or indicated times (d) under hypoxia or normoxia; whole cellular (a, b) or nucleus extracts (a) were analyzed by immunoblotting; cellular proliferation rate was examined by BrdU incorporation assay (c) or cell number accounting (d). In a, b, immunoblotting analyses were performed using the indicated antibodies. In c, d, the values are presented as mean ± s.e.m. (n = 3 independent experiments), *represents p < 0.05 and **represents p < 0.01 (Student’s t-test) between the indicated groups.