Fig. 5: PFKFB3-S172 phosphorylation inhibits cell proliferation under hypoxia.
From: O-GlcNAcylation of PFKFB3 is required for tumor cell proliferation under hypoxia
a–d SW1990 cells were expressed with a vector for control shRNA or PFKFB3 shRNA and reconstituted with expression of rPFKFB3 WT or rPFKFB3 S172A. SW1990 cells expressing with indicated shRNA and indicated Flag-PFKFB3 were cultured for 12 h under hypoxia or normoxia; immunoprecipitation analyses (a) and immunoblotting (a–c) were performed. d SW1990 cells expressing with indicated shRNA and indicated Flag-PFKFB3 were cultured for 12 h under hypoxia or normoxia; cellular proliferation rate was examined by BrdU incorporation assay and FACS analyses. In a–c, immunoblotting analyses were performed using the indicated antibodies. In d, the values are presented as mean ± s.e.m. (n = 3 independent experiments), *represents p < 0.05 (Student’s t-test) between the indicated groups.
