Fig. 2: MiR-93 depletion modifies the phenotype and behavior of pancreatic cancer cells. | Oncogenesis

Fig. 2: MiR-93 depletion modifies the phenotype and behavior of pancreatic cancer cells.

From: MiR-93 is related to poor prognosis in pancreatic cancer and promotes tumor progression by targeting microtubule dynamics

Fig. 2

a Schematic representation of the miR-106b-25 cluster and the CRISPR/Cas9 strategy for miR-93 depletion in PANC-1 cells. b MiR-93 expression analysis by qRT-PCR in PANC-1 control or KO-miR-93 cells, n = 2. c Optical microscope images. Scale bars 10 µm, magnitude 10×. d Doubling time of PANC-1 cells with or without miR-93 in hours, n = 3; C1: clone1 and C2: clone2. e MTS assay to show the effects of miR-93 depletion on PANC-1 cell proliferation and viability (n = 3). f Transwell invasion assay of PANC-1 KO-miR-93 cells compared with PANC-1 Control cells (n = 3). Statistics for number of invading cells per insert, representative images of the analyzed conditions magnitude 4×, scale bars 100 µm. Error bars = s.d.; *p ≤ 0.05 **p ≤ 0.01; ***p ≤ 0.001.

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