Fig. 4: Loss of COX6B2 further reduces oxygen consumption, ATP levels, and MMP levels. | Oncogenesis

Fig. 4: Loss of COX6B2 further reduces oxygen consumption, ATP levels, and MMP levels.

From: COX6B2 drives metabolic reprogramming toward oxidative phosphorylation to promote metastasis in pancreatic ductal cancer cells

Fig. 4

a–c Oxygen consumption rate (OCR) of SW1990, PANC-1, PaTu-8988t cells with knockdown of COX6B2 and paired control cells using the Seahorse XF24 flux analyzer after successive addition of oligomycin (1 μM), FCCP (0.5 μM), and rotenone/antimycin A (0.5 μM/0.5 μM). d Total intracellular ATP levels in cultured SW1990, PANC-1, PaTu-8988t cells with knockdown of COX6B2 and paired control cells. Total intracellular ATP was measured using bioluminescence assays. e Mitochondrial ATP levels in SW1990, PANC-1, PaTu-8988t cells with knockdown of COX6B2 and paired control cells. Mitochondrial ATP was measured using bioluminescence assays. f Total intracellular ATP and mitochondrial ATP levels of COX6B2 KD PaTu-8988t cells with re-expression of COX6B2 and paired control cells. g MMP levels of SW1990 (first panel), PANC-1 (second panel), PaTu-8988t (third panel) cells with knockdown of COX6B2 and re-expression of COX6B2 in PaTu-8988t cell with knockdown of COX6B2 (fourth panel) compared with corresponding control cells. Representative photomicrographs were captured using a fluorescence microscope (400× magnification, scale bar = 25 μM). h Representative fluorescence photomicrographs captured using a confocal laser microscope (600× magnification, scale bar = 25 μM) in COX6B2 KD PaTu-8988t cells compared with control cells. Cells were stained with 2 μM BODIPY 581/591 C11 in serum-free DMEM for 30 min. All data are presented as mean ± SEM (n ≥ 3). *P < 0.05, **P < 0.01, and ***P < 0.001.

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