Fig. 4: Carbidopa promotes translocation of AHR protein to nucleus and also increases AHR mRNA levels in LNCaP cells. | Oncogenesis

Fig. 4: Carbidopa promotes translocation of AHR protein to nucleus and also increases AHR mRNA levels in LNCaP cells.

From: Carbidopa suppresses prostate cancer via aryl hydrocarbon receptor-mediated ubiquitination and degradation of androgen receptor

Fig. 4

a Immunofluorescence staining to determine subcellular localization of AR. LNCaP cells were treated with or without Carbidopa (100 μM) for 24 h. Scale bars = 50 μm. b RT-PCR analysis of AHR, ARNT, and CYP1A1 mRNA levels. LNCaP cells were treated with or without Carbidopa (100 μM) for 24 h. c qRT-PCR analysis of the mRNA level of AHR, ARNT, and CYP1A1. LNCaP cells were treated with or without Carbidopa. GAPDH was used as the loading control. Results were normalized to control and data are given as means ± SEM of three independent experiments. *p < 0.05 versus control. **p < 0.01 versus control. d Immunoblot analysis of AHR protein. LNCaP cells were treated with or without Carbidopa (100 μM) for 24 h. e Quantitative analysis of each immunoblot; results were normalized to control and data are given as means ± SEM of three independent experiments. *p < 0.05 versus control.

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