Fig. 1: Expression of ALOXE3 is down-regulated in human GBM. | Oncogenesis

Fig. 1: Expression of ALOXE3 is down-regulated in human GBM.

From: miR-18a promotes glioblastoma development by down-regulating ALOXE3-mediated ferroptotic and anti-migration activities

Fig. 1

A GEPIA analysis of ALOXE3 mRNA level in open access datasets (n = 207 for normal brain tissues and n = 163 for GBM). B, C Human GBM tissues and normal brain tissues were used (n = 6). B Relative mRNA level of ALOXE3 normalized with GAPDH in human GBM tissues and normal brain tissues. The mRNA level is expressed as fold change over normal brain tissues. C Immunoblotting analysis of ALOXE3 and GAPDH in human GBM tissues and normal brain tissues. Representative immunoblot images are shown. The bar chart is the relative expression level of ALOXE3 normalized with GAPDH. D Immunohistochemistry staining of ALOXE3 in human GBM tissue microarray (n = 8 for normal brain tissue and n = 19 for GBM). E Diagram of arachidonic acid metabolism catalyzed by ALOXE3. F, G Circulating levels of (F) 12-KETE and (G) 12-HETE in the cerebrospinal fluid of GBM patients (n = 10). All data are represented as the mean ± s.e.m. *p < 0.05 (Student’s t-test).

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