Fig. 4: p53 activates lipoxygenase activity of ALOXE3 through suppressing SLC7A11. | Oncogenesis

Fig. 4: p53 activates lipoxygenase activity of ALOXE3 through suppressing SLC7A11.

From: miR-18a promotes glioblastoma development by down-regulating ALOXE3-mediated ferroptotic and anti-migration activities

Fig. 4

A Immunoblotting analysis of p53, SLC7A11, ALOXE3, and GAPDH in shALOXE3-U87 and shCont-U87 cells with or without Nutlin-3a treatment. Representative immunoblot images are shown (n = 6). B, C Levels of (B) 12-KETE and (C) 12-HETE in culture medium harvested from shALOXE3-U87 and shCont-U87 cells with or without Nutlin-3a treatment (n = 6). D Western blot analysis of the endogenous interaction between ALOXE3 and SLC7A11 in U87 cells. E Western blot analysis of the endogenous interaction between ALOXE3 and SLC7A11 in U87 cells treated with siSLC7A11-1 or siCont. F 12-KETE levels of culture medium harvested from shALOXE3-U87 and shCont-U87 cells transfected with siSLC7A11-1 or siCont (n = 6). G Mechanistic insight into p53-mediated activation of ALOXE3 activity and its associated ferroptosis. All data are represented as the mean ± s.e.m. *p < 0.05 (Student’s t-test).

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