Fig. 1: JMJD3 is overexpressed in Ras-activated lung cancer cells.
From: Epigenetic regulation of TGF-β-induced EMT by JMJD3/KDM6B histone H3K27 demethylase
a Relative expression of JMJD3 in lung tissues from lung cancer patients and normal (normal n = 27, lung cancer n = 27). ***P < 0.001 versus normal. b Overall survival rate curve of lung cancer patients according to JMJD3 expression (low n = 484, high n = 1442). c Correlative expression of JMJD3 compared to K-ras in lung tissues. d Correlative expression of JMJD3 compared to H-ras in lung tissues. e Real-time RT-PCR (left panel) and western blot analysis (right panel) of JMJD3 expression levels in BEAS-2B cells transfected with K-RasV12 or H-RasV12 expression vector. Columns, mean of three independent experiments performed in triplicate; bar, S.D.; **P < 0.01 versus control. f Real-time RT-PCR (left panel) and western blot analysis (right panel) of JMJD3 expression levels in BZR cells transfected with control or H-RasN17 expression vector. Columns, mean of three independent experiments performed in triplicate; bar, S.D.; **P < 0.01 versus control. g Real-time RT-PCR (left panel) and western blot analysis (right panel) of JMJD3 expression levels in normal (BEAS-2B cells) and Ras-activated lung cancer cells (A549, H358, H1299, and BZR cells). Columns, mean of three independent experiments performed in triplicate; bar, S.D.; *P < 0.01 versus BEAS-2B. h Western blot analysis of H3K27me3 in BEAS-2B, A549, H358, H1299, and BZR cells. i Immunofluorescence analysis of expression and localization of JMJD3 (green) in A549 cells treated with DMSO, LY294002 (10 μM), or U0126 (10 μM). DAPI-staining nuclei (blue) were also shown. Scale bar, 20 μm. j Expression of JMJD3 in cytosolic or nuclear fractions of A549 cells treated with DMSO, LY294002 (10 μM), or U0126 (10 μM). PARP (nucleus) and tubulin (cytoplasm) were also shown. k Expression level of JMJD3 in BZR, A549, and H1299 cells transfected with the increasing amount of DN-MEK1 expression vector. l Expression level of JMJD3 in BZR, A549, and H1299 cells transfected with the increasing amount of DN-AKT1 expression vector.
