Fig. 5: JMJD3 binds to syntenin promoter and demethylates H3K27me3.
From: Epigenetic regulation of TGF-β-induced EMT by JMJD3/KDM6B histone H3K27 demethylase
a ChIP assay of JMJD3 (upper panel) and H3K27me3 (lower panel) recruitment at syntenin promoter using IgG, JMJD3, or H3K27me3 antibody in H-RasV12-expressed BEAS-2B cells. Columns, mean of two independent experiments performed in triplicate; bar, S.D.; ***P < 0.001 versus control in each group, n.s.: nonsignificant. b ChIP assay of JMJD3 (upper panel) and H3K27me3 (lower panel) recruitment at syntenin promoter using IgG, JMJD3, or H3K27me3 antibody in H-RasN17-expressed BZR cells. Columns, mean of two independent experiments performed in triplicate; bar, S.D.; **P < 0.01, ***P < 0.001 versus control in each group, n.s.: nonsignificant. c ChIP assay of JMJD3 (upper panel) and H3K27me3 (lower panel) recruitment at syntenin promoter using IgG, JMJD3, or H3K27me3 antibody in HA-JMJD3-expressed BEAS-2B cells. Columns, mean of two independent experiments performed in triplicate; bar, S.D.; ***P < 0.001 versus control in each group, n.s.: nonsignificant. d ChIP assay of JMJD3 (upper panel) and H3K27me3 (lower panel) recruitment at syntenin promoter using IgG, JMJD3, or H3K27me3 antibody in BZR cells in response to JMJD3 knockdown. Columns, mean of two independent experiments performed in triplicate; bar, S.D.; ***P < 0.001 versus control in each group, n.s.: nonsignificant. e ChIP assay of JMJD3 (upper panel) and H3K27me3 (lower panel) recruitment at syntenin promoter using IgG, JMJD3, or H3K27me3 antibody in A549 cells in response to JMJD3 knockdown. Columns, mean of two independent experiments performed in triplicate; bar, S.D.; *P < 0.05 versus control in each group, n.s.: nonsignificant. *P < 0.05 versus control in each group, n.s.: nonsignificant.
