Fig. 4: Inhibition of oncogenic effects of ETS1 by EHF-SF.

A, B, and C HOC313 and TSU cells infected with lentiviruses carrying control (cont.) or flag-tagged EHF-SF were subjected to IB analysis using the indicated antibodies (A). Motility assays using a Boyden chamber assay with inserts coated with type I collagen gel (upper panels) and a wound healing assay (lower panels) (B). Chemoresistance assay in response to the indicated concentration (conc.) of docetaxel (C). Each value represents the mean ± s.d. of triplicate determinations from a representative experiment. Similar results were obtained in at least three independent experiments. D and E HSC3 cells infected with either control (n = 6) or flag-tagged EHF-SF (n = 8) were injected into the periosteal region of the parietal bone in mice. Five weeks later, mice were sacrificed, and tumor volume was measured (E). After decalcification, specimens were prepared. Typical histology in hematoxylin and eosin staining are shown. B bone, T tumor. (F, G, H, I, and J) MDA-MB-231-Luc cells infected with either control or EHF-SF were visualized by phase-contrast microscopy (F), and subjected to IB analysis (G) and motility assays (H). The cells were injected into the left ventricle of the heart, followed by imaging analysis (I) and quantification (J) at 35 days (5 weeks). p values were determined by Student’s t-test. *p < 0.01, **p < 0.03. α-tubulin was used as a loading control (A and G).