Fig. 1: Generation of murine gall bladder (GB) organoids with KrasG12D and p53 loss. | Oncogenesis

Fig. 1: Generation of murine gall bladder (GB) organoids with KrasG12D and p53 loss.

From: Precision modeling of gall bladder cancer patients in mice based on orthotopic implantation of organoid-derived tumor buds

Fig. 1

A Schematic view of the experimental design. TSG tumor suppressor gene, KO knockout, WT wild type. B Immunohistochemical staining of organoids. Korg, organoids expressing KrasG12D; K/53org, Korg with Trp53 knockout. Scale bar, 100 μm. C The proliferation rate in organoid culture. Mean cell count of six wells ± SD is shown. *, significant (p < 0.05). D–F Flow cytometry analysis of K/53org and Korg at day 4 in C. D Ki-67 expression profiles. Left panel displays a representative histogram. Right panel displays a summary of the results from replicates (n = 5). MFI median fluorescent intensity. E Density plots of cell viability analysis. Representative images are shown. Among the phosphatidylserinehigh population, viability dyelow fraction and viability dyehigh fraction were interpreted as apoptotic and dead cells, respectively. *, significant (p < 0.05). F Summary of the results from replicates (n = 5) in E. *, significant (p < 0.05).

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