Fig. 2: Subcutaneous tumor development from gall bladder (GB) organoids in syngeneic mice. | Oncogenesis

Fig. 2: Subcutaneous tumor development from gall bladder (GB) organoids in syngeneic mice.

From: Precision modeling of gall bladder cancer patients in mice based on orthotopic implantation of organoid-derived tumor buds

Fig. 2

A Macroscopic view of a subcutaneous tumor in a WT C57BL/6 J mouse. A representative image of a subcutaneous tumor (open arrowhead) derived from injected K/53org is shown. B Neo-angiogenesis in the tumor. Induction of surrounding blood vessels (closed arrowhead) into the tumor (open arrowhead) is shown. C An isolated subcutaneous tumor. Scale bar, 5 mm. D Histological findings and immunohistochemical staining of K/53org subcutaneous tumor. Note that hyalinization of the stroma (asterisk) is indicative of central necrosis. Scale bar, 100 μm. E Immunofluorescence staining of tumor vessels. Left, vascular endothelial cells (red, CD31) and pericytes (green, alpha smooth muscle actin [α-SMA]) are stained. The nucleus (blue) is visualized by DAPI. Scale bar, 500 μm. Right, vessels outside the tumor covered by α-SMA-positive cells (arrowhead) and uncovered vessels in the tumor (arrow). Scale bar, 100 μm. F Histological findings of subcutaneous tumors. K/Smad4org, Korg with Smad4 knockout. K/p19Arforg, Korg with p19Arf knockout. Scale bar, 500 μm (low magnification), 100 μm (high magnification).

Back to article page